These notes were made for Biochem 3381 at the University of Western Ontario and cover secondary structure, quaternary structure, protein purification, and miscellaneous biochemistry techniques.
Ramchandran plots and some secondary structure
Quaternary Structure
Misc Techniques
Protein Purification
Bonus! Code in R for generating Ramchandran plots like this one: Ramchandran plot
phi <- c(value,value,value…)
psi <- c(value,value,value…)
residues <- c(“number, residue”,”number, residue”,”number, residue”…)
par(pty=”s”)
plot(x=phi, y=psi,
xlim=c(-180,180),ylim=c(-180,180), axes=FALSE,
main=”Your title”,
pch=20, xlab=expression(phi),
ylab=expression(psi), cex=1, asp=1.0)
axis(1, at=seq(-180,180,90))
axis(2, at=seq(-180,180,90))
text(phi, psi, labels=residues, cex= 0.7,pos=3)
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